ABSTRACT
Objective To investigate the effect of lysimachia capillipes(LC) on proliferation of human pancreatic cancer cell line BxPC-3 in vitro and explore the potential mechanism.Methods BxPC-3 cells were treated by LC in different concentrations of 2,4,8,16,32,64 μg/ml for 48 and 72 hours,respectively,using untreated cells as control.The survival rate of BxPC-3 cells was measured by MTT method.The half inhibition concentration (IC50) of LC was determined by drawing growth curve.BxPC-3 cells were treated by LC in the concentration of IC50 (LC group),and cell apoptosis and cell cycle were examined by using flow cytometry.The protein expression of PARP and capase-3 was detected by Western blotting.Results LC in the concentration of 8-32 μg/ml inhibited the survival rate of BxPC-3 cells in a dose-dependent manner.After exposure to 15 μg/ml LC for 48 h,the apoptosis rate of BxPC-3 cells was increased [(17.3 ± 0.31)％ vs (1.5 ± 0.22) ％],but the cell cycle was not affected.The expression of caspase-3 protein was up-regulated [(2207.2 ± 92.0) vs 149.1 ± 10.2] and PARP protein was down-regulated [(36.1 ± 4.8) vs 1593.4 ±29.7] than control group,and the differences were statistically different (all P value ＜ 0.05).Conclusions LC can inhibit the growth of BxPC-3 cells,and the potential mechanism was associated with the induction of cell apoptosis by LC via upregulating caspase-3 protein expression and decompsing PARP protein.
ABSTRACT
Objective To discuss necessity of cholecystectomy for patients with stones in their bile ducts and gallbladders in the absence of absolute operation indications of cholecystectomy after clearance of bile duct stones with ERCP. Methods Data of less than 70-year-old patients with stones in their bile ducts and gallbladders but without absolute operation indications of cholecystectomy admitted to the First People′s Hospital of Hangzhou from January 2012 to December 2014 were retrospectively analyzed. Patients were divided into cholecystectomy group and gallbladders in situ group after bile duct stones removal with ERCP. The hospitalization time, recurrent biliary events, complications of operation were compared between two groups. Results A total of 128 patients were included and followed up for(24.031±9.170)months with 64 cases in conservative group and 64 cases in cholecystectomy group. Incidence of recurrent biliary events in the cholecystectomy group and conservative group were 7.8%(5/64)and 10.9%(7/64)(P=0.544). Among cases of recurrent biliary events,incidences of cholangitis in the two groups were 7.8%(5/64)and 4.7%(3/64)respectively(P= 0.465), incidences of pancreatitis were 0(0/64)and 1.6%(1/64) respectively(P=0.315), incidences of common bile duct stones were 7.8%(5/64)and 7.8%(5/64)respectively(P = 1). No recurrent cholecystitis occurred. There were no differences in ERCP-related complications between the cholecystectomy group[23.4%(15/64)]and the conservative group[12.5%(8/64)](P=0.107). But 4 patients(6.3%)in cholecystectomy group had LC-related complications during the follow-up period, 3 cases of infection and 1 ileus. The hospital stay in conservative group was shorter than that in cholecystectomy group(5.906± 4.614 days VS 14.313± 6.312 days, P<0.001). The hospitalization expenses in two groups were(45 196± 12 518)yuan and(22 160± 11 755)yuan(P<0.001). Conclusion Conservative methods yield to shorter hospitalization and lower expenses without cholecystectomy-related complications. Conservative management for gallbladder stones after ERCP for biliary duct stones is recommended for patients who had no absolute operation indications of cholecystectomy.